The main difference between leading and also lagging strand is the the top strand is the DNA strand, which grow continuously during DNA replication conversely, lagging strand is the DNA strand, which grows discontinuously through forming brief segments well-known as Okazaki fragments. Therefore, to type a continuous strand, the top strand go not require ligase if the lagging strand calls for ligase to ligate Okazaki pieces together. Furthermore, the top strand opens in the 3’ to 5’ direction while the lagging strand opens up in the 5’ come 3’ direction.

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Leading and lagging strand are two terms the we usage to explain the two strands of the double-stranded DNA during DNA replication based upon the pattern of strand growth.

Key areas Covered

1. What is a top Strand – Definition, type of Strand Growth, Importance 2. What is a Lagging Strand – Definition, type of Strand Growth, Importance 3. What are the Similarities between Leading and also Lagging Strand – overview of typical features 4. What is the Difference in between Leading and Lagging Strand – compare of crucial Differences

Key Terms

DNA replication, top Strand, Lagging Strand, Okazaki Fragments, Replication Fork

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What is a top Strand

Leading strand is among the two strands that the DNA dual helix. Generally, DNA experience replication during the cabinet cycle as a action of prepare the cell for the division. DNA polymerase is the enzyme the is responsible for DNA replication lugged out specifically in the 5’ come 3’ direction. During the process, every strand the the DNA twin helix serves as templates for replication. Therefore, the procedure of DNA replication is known as a semiconservative process where each freshly synthesized DNA twin helix composes an old and a new DNA strand


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Figure 1: DNA Replication.


During replication, the DNA twin helix unwinds to form the replication fork. Here, the DNA strand, which opens up in the 3’ to 5’ direction enables the expansion of the strand continuously in the 5’ come 3’ direction. Therefore, we speak to this strand together the top strand. In the leading strand, DNA polymerase can add nucleotides continuously, and also the growth of the brand-new DNA strand occurs towards the replication fork.

What is a Lagging Strand

The lagging strand is the second strand of the DNA double helix. The strand opens up in the 5’ to 3’ direction. Therefore, the brand-new strand growth has to take place away indigenous the replication fork together the direction of DNA replication occurs just in the 5’ come 3’ direction. On the account, the replication process is no continuous, and it occurs through the development of Okazaki fragments. Generally, Okazaki pieces are quick segments that DNA, about 1000-2000 nucleotides long.


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Figure 2: continuous of the Leading and Lagging Strand


Also, in ~ the beginning of each Okazaki fragment, one RNA primer needs to be synthesized ~ above the lagging strand. RNA primase is the enzyme responsible for the synthetic of RNA primers top top the layout DNA during DNA replication. The development of the last Okazaki fragment stops at the 5’ finish of the RNA inside wall of the previous Okazaki fragment. Significantly, the DNA replication that the lagging strand always has a ‘wait time’ because that the synthetic of a new RNA primer. Ultimately, the RNA primers are eliminated from the strand and DNA polymerase fills the absent nucleotides. Then, DNA ligase joins every DNA fragment together on the lagging strand, making a consistent DNA strand.


Similarities in between Leading and also Lagging Strand

Leading and lagging strand room the two types of DNA strands discovered in the double-stranded DNA molecule.They space classified based upon the pattern of replication.However, the leading and also the lagging strand room complementary to each other.Furthermore, both strands are consisted of of DNA nucleotides, which connect to each various other through phosphodiester bonds.Also, DNA polymerase is responsible for the synthetic of both leading and also lagging strands.

Difference in between Leading and also Lagging Strand

Definition

Leading strand advert to one of two strands of DNA found at the replication fork, gift replicated repeatedly while lagging strand describes the various other strand found at the replication fork, replicating discontinuously in the 5′ to 3′ direction. Thus, this is the key difference between leading and lagging strand.

Type the Growth throughout DNA replication

Importantly, the top strand grows consistently while the lagging strand grow discontinuously by creating Okazaki fragments.

The direction the the template in the Replication Fork

Furthermore, another important difference in between leading and lagging strand is the the leading strand opens up up in the 3’ to 5’ direction when the lagging strand opens up up in the 5’ come 3’ direction.

The direction the Strand Growth

Leading strand grow in the 5’ to 3’ direction when the lagging strand grows in the 3’ to 5’ direction.

Primers

Leading strand requires a single primer because that the synthesis while the lagging strand requires a new primer to begin each Okazaki fragment. Hence, this is another difference in between leading and lagging strand.

The beginning of the Replication

Moreover, the starting point also contributes come the difference between leading and also lagging strand. The top strand beginning to flourish at the start of replication when the lagging strand starts to replicate quickly after.

Direction from the Replication Fork

Also, the top strand grows in the direction of the replication fork if the Okazaki pieces of the lagging strand flourish away from the replication fork.

Speed that Formation

Additionally, the speed of formation is another difference in between leading and also lagging strand. The development of the leading strand occurs at high rate while the formation of the lagging strand occurs slowly.

The need of DNA Ligase

Besides these, the top strand go not need DNA ligase when the lagging strand calls for DNA ligase to ligate Okazaki fragments together.

Conclusion

Leading strand is one of the 2 strands that the double-stranded DNA. Significantly, it opens up up in the 3’ to 5’ direction in ~ the replication fork. Therefore, it undergoes strand expansion continuously in the 5’ come 3’ direction during the DNA replication. In comparison, the lagging strand is the other strand in the DNA twin helix. However, it opens up in the 5’ to 3’ direction. Therefore, its strand expansion has to occur in the 3’ come 5’ direction. But, normal DNA replication wake up in the 5’ to 3’ direction only. Hence, DNA replication occurs in the direction of the exterior of the replication fork discontinuously by developing Okazaki fragments. On the account, the key difference between leading and lagging strand is the direction and the sample of strand growth.

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References:

1. “What Is DNA Replication?” yourgenome, the public Engagement Team at the Wellcome Genome Campus, 25 Jan. 2016, available Here.

Image Courtesy:

1. “DNA replication en” by LadyofHats Mariana Ruiz – very own work. Image renamed indigenous File:DNA replication.svg (Public Domain) via Commons Wikimedia 2. “Timeline of replication for leading and lagging strand.” through Thermodynamic – Own work (CC BY-SA 3.0) via Commons Wikimedia